产品货号：
GS2177
中文名称：
Bst6I内切酶
英文名称：
Bst6I
产品规格：
200U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
To obtain 100%activity,BSA should be added to the 1x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.
技术规格
相关搜索：Bst6I内切酶，Bst6I限制性内切酶
使用说明
To obtain 100%activity,BSA should be added to the 1x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1hourat 65℃ in a total reaction volume of 50μl. |
| Recognition sites | CTCTTC(N)1↑ |
| 来源 | Bacillus stearothermophilus 6 |
| Assayed on | Lambda DNA |
| Optimal buffer | Y(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.)+ BSA |
| Optimal temperature | 65℃ |
| Storage conditions | 10 mM Tris-HCl(pH 7.5);50 mM KCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol.|For long term storage(more than 30 days),store at -70℃. |
| Ligations | After 2-fold overdigestion with enzyme 80%of DNA fragments can be ligated.Of these 80%can be recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a.of enzyme for 16hoursat 65℃. |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 80℃ for 20 mins. |
| Campatible Ends | Eam1104I,EarI,Ksp632I |
相关搜索：Bst6I内切酶，Bst6I限制性内切酶